•MIF functions as a proinflammatory cytokine involved in most inflammatory reactions, as well as promotes the release of insulin. These characteristics are what make MIF a key part in the onset of diabetes, and a producer of cancer symptoms.
•Recent research suggests that isoxazolines not only shield ß-cells in the pancreas (thus combating diabetes), but also inhibit MIF by binding to its catalytic sites and lowering its protein production.
•More recent research suggests that an ideal way to synthesize isoxazolines involves a Grignard reaction of p-anisaldehyde with a metalloid followed by oxidation of an alcohol with the 3-buten-1-ol functional group with pyridinium chlorochromate (PCC) to form a ketone with the functional group 3-buten-1-one. The ketone is then treated with a hydroxylamine and a form of acetate. The final synthesis involves a cyclization with a metalloid to close the isoxazole ring, thus creating a form of isoxazoline.
•These synthesis reactions have proven to produce high yields, and are fairly cost-efficient.